Desarrollo De Lineas De Habichuela (phaseolus Vulgaris L.) Adaptadas A Las Condiciones Ecologicas De Haiti Con Resistencia A Enfermedades E Insectos

Resumen del Libro

The release of bean varieties (Phaseolus vulgaris L.) with resistance to diseases and pests could reduce yield losses in Haiti. Marker-assisted selection and conventional methods of bean improvement were used to develop lines with resistance to Bean Golden Yellow Mosaic Virus (BGYMV), Bean Common Mosaic Necrotic Virus (BCMNV), powdery mildew caused by [Erysiphe polygoni (DC ex-Merat)] and the Mexican bean weevil [Zabrotes subfasciatus (Boheman)]. Sources of resistance from Haiti, Puerto Rico, the International Center of Tropical Agriculture, Zamorano and the University of Wisconsin were used as progenitors. F 2 bean plants were evaluated in the greenhouse for resistance to natural infection of powdery mildew. F2:3 and F3:4 lines were evaluated in the field in Salagnac, Haiti for adaptation and reaction to natural infection of diseases. We used the SCAR marker SR2 to detect F2:3 and F3:4 lines with the bgm gene for resistance to BGYMV. The same plants were inoculated with BCMNV NL3 strain to identify plants with resistance to the virus. We identified six red mottled lines with BGYMV, BCMNV and powdery mildew resistance. Only two of these lines, however, produced seed yields similar to Salagnac 90A. The SCAR marker SR2 and SDS PAGE were used to identify red mottled, white and red lines with resistance to the BGYMV and the Mexican bean weevil. We identified 12 F 2 plants that were homozygous for the bgm gene for BGYMV resistance and Arcelin 1 that confers resistance to the weevils. F 2:3 lines derived from F2 plants heterozygous for BGYMV or Arcelin will be advanced for additional evaluations. Lines with multiple resistance will be used by the bean improvement programs of Haiti and Puerto Rico as improved breeding and parental lines in crosses.